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KMID : 0380020060210040292
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.292 ~ p.297
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Effect of Phellinus Extracts on Sprouting in Porcine Pulmonary Artery Endothelial Cells
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Oh In-Suk
Kim Hwan-Gyu
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Abstract
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One of the steps in angiogenesis is the degradation of the underlying basement membrane via proteases. Endothelial cells release proteinases to degrade the extracellular matrix for their sprouting in vivo. In this study, we examined the effect of water extracts of Phellinus linteusis (Phellinus extracts) and combination of Phellinus extracts and fibroblast growth factor (FGF-2) on cultured porcine pulmonary artery endothelial cells (PPAECs). Phellinus extracts induced sprouting of PPAECs, which was inhibited by MMPs and plasmin inhibitors, and induced the secretion of matrix metalloproteinase-3 (MMP-3) and plasmin. At high concentration of Phellinus extracts (200~400 μg/mL), the active MMP-2 secretion was induced. It is therefore, suggested that Phellinus extracts induces the sprouting of cultured endothelial cells by means of increased active MMP-2 and plasmin secretion. Also, combination with Phellinus extracts and FGF-2 produced an enhanced effect on sprouting and secretion of active MMP-2, and MMP-3 and plasmin from PPAECs.
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KEYWORD
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Phellinus Linteusis, sprouting, MMP, plasmin
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